Friday, 15 February 2013

DDW memo (Lecture 1 (Part 3/3) of 1st International Deuterium Symposium)

DDW memo.

Lecture 1 (Part3/3) of 1st International Deuterium Symposium

Quote from YouTube:

Dr. Gábor Somlyai speaks about "Naturally occurring Deuterium is essential for the normal growth rate of cells"

About the symposium:
1st International Symposium on Deuterium Depletion is an international conference, wishes to give the researchers and medical practitioners a unique opportunity to get acquainted with this expanding new expanding field of science and share their results and experience.

The conference primarily focus on the biological effects of naturally occurring deuterium and its importance in cancer, diabetes and aging, but the scientific committee welcomed any other papers originating from basic research which reveal the role of deuterium in the different regulatory systems of the cells.

The basic concept of the conference is to gather all the scientists from the world, who have carried out basic or applied research on deuterium depletion, in order to share these results, experience, scientific and clinical information with the participants of the symposium and by these means to summarize this knowledge for the other scientists and clinicians involved in this field and other professional participants and share the basic rules of the application of DDW in the clinical practice.

DDW memo (Lecture 1 (Part 2/3) of 1st International Deuterium Symposium)

Part 2/3 of the Symposium.

Lecture 1 (Part2/3) of 1st International Deuterium Symposium

Quote from YouTube:

Dr. Gábor Somlyai speaks about "Naturally occurring Deuterium is essential for the normal growth rate of cells"

About the lecturer: Gábor Somlyai, Ph.D.

Gábor Somlyai graduated as a biologist at the University of Szeged in 1982. Between 1982 és 1990 he worked for the Plant Protection Institute of the Hungarian Academy of Sciences, Department of Plant Pathology, within this time period from 1983 to 1986 he was a scholarship-holder of the Hungarian Academy of Sciences as a postgraduate student for obtaining PhD. 

In 1988 he defended his thesis in molecular biology. In the same year he was a DFG-scholarship holder at Georg August University in Göttingen for 6 months, from the end of 1988 Dr. Somlyai held a postdoctoral fellowship at the University of Missouri (Columbia, Missouri, USA), where he worked in the field of genetic engineering and gene mapping. 

In the wake of Hungarian Nobel-prize winning researcher Albert Szent-Györgyi, who said that the true cause of cancer should be looked for at sub-molecular level, Somlyai began his investigations in 1990 as a senior research fellow at the Hungarian Institute of Oncology with the examination of whether the naturally occuring deuterium the heavy isotope of hydrogen has any role in the regulation of biological, i.e. intracellular molecular processes. 

In 1993 he established HYD Ltd. with his partners to carry out anticancer research based on his invention, drug development based on the proprietary procedure of deuterium depletion and to conduct drug registration.

Between 1993 and 1997 he was the scientific director of HYD Ltd., from 1997 he became the CEO of the company. 

In 2000 his book entitled "Defeating cancer!" appeared in Hungary, from that time it has been published in Romania, Japan, the USA, South-Korea and in China. 

Gábor Somlyai is the author of numerous scientific publications in Hungarian, English and German languages and recognized speaker of domestic and international conferences.

DDW memo (Lecture 1 (Part1/3) of 1st International Deuterium Symposium)

Here's a quick reblog post of 2010 International Deuterium Symposium in Hungary.  (... to sort out later.)

Lecture 1 (Part2/3) of 1st International Deuterium Symposium

Quote from YouTube:
Dr. Gábor Somlyai speaks about "Naturally occurring Deuterium is essential for the normal growth rate of cells"

About the lecture:
1) G. Somlyai, 2) G. Jancsó, 2) Gy. Jákli, 3) M. Molnár, 4) G. Laskay, 5) L.Z. Fehér, 6) L.G. Puskás 
1) HYD LLC. for Cancer Research and Drug Development, Budapest, Hungary, 
2) KFKI Atomic Energy Research Institute, Budapest, Hungary, 
3) Semmelweis University Medical School, Budapest, Hungary, 
4) University of Szeged, Department of Plant Biology, Szeged, Hungary, 
5) Avidin Ltd, Szeged, Hungary, 
6) Biological Research Center of the Hungarian Academy of Sciences, Laboratory of Functional Genomics, Szeged, Hungary.

The role of naturally occurring D in living organisms has been examined by using deuterium-depleted water (DDW) (30-100 ppm) instead of water containing the natural abundance of D (150 ppm). DDW significantly decreased the growth rate of L929 fibroblast, HT-29 colon, A4, MDA and MCF-7 breast, PC-3 prostate, M19 melanoma cell lines. 

The inhibitory effect was more significant when the D-concentration of the culture medium was gradually decreased in 3-5 steps. 

To investigate the anticancer effect of DDW in vivo, human breast adenocarcinomas; MDA and MCF-7 were transplanted into CBA/Ca mice. The drinking water of the animals in the treated group (17) was replaced with DDW (30 ppm) one day after transplantation. 

Eighty days after the transplantation all mice in the control group perished (10) except one, meanwhile 70% of the animals (12) were still alive in the treated group. In an other experiment, PC-3 tumorous prostatic cells were transplanted into CBA/Ca mice. 

The DDW treatment started on the 18th day. Twelve days later the tumors were removed from mice and histologically examined. Cells being in mitosis and apoptosis were counted. It was found that in the control group, that received water with normal (150 ppm) D-content, 3.6% of the cells were in mitosis and only 1% in apoptosis. 

The ratio was almost the opposite in the treated group, where only 1.5% of the cells were in mitosis, while 3% of the cells were in apoptosis. In order to reveal the molecular background of the inhibitory effect of DDW COX-2 gene expression was investigated in healthy myometrial and HT-29 colon tumorous cell line in medium with 20-80-150-200-500-1000 ppm D. 

It was found that deuterium depletion inhibited COX-2 expression and the inhibition correlated with the D-concentration. At the same time there was a strong correlation between the COX-2 expression and the intracellular prostaglandin concentration. When the prostaglandin was added back to the culture medium it diminished the inhibitory effect of DDW. 

The application of DDW also proved to influence the expression of genes encoding different kinases using nanocapillary quantitative real-time PCR analysis technique and to modify the activity of amilorid- sensitive Na+/H+ antiport system.

We suggest that cells are able to regulate the D/H ratio and its changes can trigger certain molecular processes. 

One possibility to modify the D/H ratio is the activation of the H+-transport system which prefers to eliminate H+ resulting in a higher D/H ratio within the cell. We suggest that the changing D/H ratio can simultaneously regulate the expression of certain genes and the activity of enzymes having key role in cell cycle regulation and in other molecular mechanisms. 

We suppose that the naturally occurring D is the key element of a hitherto unknown sub-molecular regulatory system (SMRS).